76-05-1 Fine Chemical Intermediates TFA Perfluoroacetic Acid For NMR Spectroscopy

Cas No. 76-05-1 Fine Chemical Intermediates With Name TFA

TFA is popularly used as a strong acid to remove t-butyl derived side-chain protecting groups in Fmoc peptide synthesis, and in other organic syntheses to remove the t-butoxycarbonyl protecting group.

At a low concentration, TFA is used as an ion pairing agent in liquid chromatography (HPLC) of organic compounds, particularly peptides and small proteins. TFA is a versatile solvent for NMR spectroscopy (for materials stable in acid). It is also used as a calibrant in mass spectrometry.

Trifluoroacetic acid is a highly corrosive substance. Contact of the liquid with the skin, eyes, and mucous membranes can cause severe burns, and ingestion can result in serious damage to the digestive tract. TFA vapor is highly irritating of the eyes and respiratory tract, and inhalation of high concentrations can lead to severe destruction of the upper respiratory tract and may be fatal as a result of pulmonary edema. Symptoms of overexposure to TFA vapor include a burning feeling, coughing, headache, nausea, and vomiting.

Trifluoroacetic acid has not been found to be carcinogenic or to show reproductive or developmental toxicity in humans.

Trifluoroacetic acid should be handled in the laboratory using the "basic prudent practices". In particular, trifluoroacetic acid should be stored in an acid cabinet away from other classes of compounds. Because of its high vapor pressure, fumes of trifluoroacetic acid can destroy labels on other bottles if the container is not tightly sealed.