Isotope Markers Acetophenone Liquid 98-86-2 Aromatic Hydrocarbons

Isotope Markers Acetophenone Liquid As Aromatic Hydrocarbons

Degradation of the aromatic ketone acetophenone is initiated by its carboxylation to benzoylacetate catalyzed by acetophenone carboxylase (Apc) in a reaction dependent on the hydrolysis of two ATP to ADP and Pi. Apc is a large protein complex which dissociates during purification into a heterooctameric Apc(αα′βγ)2 core complex of 482 kDa and Apcε of 34 kDa. In this report, we present the X-ray structure of the Apc(αα′βγ)2 core complex from Aromatoleum aromaticum at ca. 3 Å resolution which reveals a unique modular architecture and serves as model of a new enzyme family. Apcβ contains a novel domain fold composed of two β-sheets in a barrel-like arrangement running into a bundle of eight short polyproline (type II)-like helical segments. Apcα and Apcα′ possess ATP binding modules of the ASKHA superfamily integrated into their multidomain structures and presumably operate as ATP-dependent kinases for acetophenone and bicarbonate, respectively. Mechanistic aspects of the novel carboxylation reaction requiring massive structural rearrangements are discussed and criteria for specifically annotating the family members Apc,  carboxylase and hydantoinase are defined.

Acetophenone represents a methyl ketone consisting of chemicals ({eq}CH_3COCH_3 {/eq}) with one methyl group replaced by a phenyl functional group. The chemical formula for acetophenone is {eq}C_8H_8O {/eq} and the structural formula is {eq}C_6H_5COCH_3 {/eq}. The IUPAC name for this compound is 1-phenylethanone and is also referred to by the following synonyms: methyl phenyl ketone and acetyl benzenes. Acetophenone is colorless and often occurs as an oily liquid when extracted from natural sources or when industrially produced.